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Kv12.3

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Not curated in GtoImmuPdb

Target id: 577

Nomenclature: Kv12.3

Family: Voltage-gated potassium channels (Kv)

Annotation status:  image of a blue circle Annotated and reviewed, awaiting update  » Email us

Contents:

Gene and Protein Information Click here for help
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 1017 17q21.2 KCNH4 potassium voltage-gated channel subfamily H member 4
Mouse 6 1 1018 11 D Kcnh4 potassium voltage-gated channel, subfamily H (eag-related), member 4
Rat 6 1 1017 10q31 Kcnh4 potassium voltage-gated channel subfamily H member 4
Previous and Unofficial Names Click here for help
BEC2 | Elk1 | brain-specific eag-like channel 2 | ELK channel 1 | ether-a-go-go-like potassium channel 1 | potassium voltage-gated channel, subfamily H (eag-related), member 4 | potassium channel, voltage gated eag related subfamily H, member 4 | potassium voltage-gated channel
Database Links Click here for help
Alphafold Q9UQ05 (Hs), Q9R1T9 (Rn)
CATH/Gene3D 2.60.120.10
Ensembl Gene ENSG00000089558 (Hs), ENSMUSG00000035355 (Mm), ENSRNOG00000018790 (Rn)
Entrez Gene 23415 (Hs), 142984 (Mm), 114032 (Rn)
Human Protein Atlas ENSG00000089558 (Hs)
KEGG Gene hsa:23415 (Hs), mmu:142984 (Mm), rno:114032 (Rn)
OMIM 604528 (Hs)
Pharos Q9UQ05 (Hs)
RefSeq Nucleotide NM_012285 (Hs), NM_001081194 (Mm), NM_053630 (Rn)
RefSeq Protein NP_036417 (Hs), NP_001074663 (Mm), NP_446082 (Rn)
UniProtKB Q9UQ05 (Hs), Q9R1T9 (Rn)
Wikipedia KCNH4 (Hs)
Ion Selectivity and Conductance Click here for help
Species:  Rat
Macroscopic current rectification:  Ik
References:  1
Voltage Dependence Click here for help
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  -59.0 38.0 1 CHO cells. Rat
Inactivation  - -
Deactivation  - 200.0 1
Comments  τ deactivation at -80mV [1]
Gating Inhibitor Comments
Voltage clamp of Kv12.3 heterologously expressed in HEK293 cells demonstrated that external H+ is a gating inhibitor with a pKa of 7.1 and holding voltage -60mV [2].
Channel Blocker Comments
At millimolar concentrations, Ba2+ acts as a pore blocker for Kv12.3 [1].
Kv12.3 is resistant to block by external TEA, 4-AP, E-4031 [1].
Tissue Distribution Click here for help
Cerebral cortex, occipital pole, fromtal lobe, tenporal lobe, putamen, caudate nucleus, hippocampus.
Species:  Human
Technique:  Northern blot
References:  3
Piriform cortex, olfactory tubercle, cerebral cortex layers II-VI, hippocampus, basal ganglia
Species:  Rat
Technique:  In situ hybridisation
References:  4
Pituitary and clonal somato-mammotroph cells (GH3 / B6 cells)
Species:  Rat
Technique:  RT-PCR
References:  5
Lung, spinal cord, hippocampus, cortex, striatum, pituitary, medulla
Species:  Rat
Technique:  RT-PCR
References:  1
Brain, testes
Species:  Rat
Technique:  Northern blot
References:  1
Functional Assays Click here for help
Membrane currents recorded from heterologously expressed channels using whole-cell patch clamp
Species:  Rat
Tissue:  CHO (Chinese Hamster Ovary) cells
Response measured:  Remarkably low threshold of activation, may contribute to the maintenance of the resting potential. Activation kinetics not dependent on prepulse potential.
References:  1
General Comments
This channel has:
  • GFG (rather than the common GYG) potassium channel signature sequence
  • a PAS domain in the distal part of the cytosolic N-terminus
  • a cNBD domain in the proximal portion of the C-terminus

References

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1. Engeland B, Neu A, Ludwig J, Roeper J, Pongs O. (1998) Cloning and functional expression of rat ether-à-go-go-like K+ channel genes. J Physiol (Lond.), 513 ( Pt 3): 647-54. [PMID:9824707]

2. Kazmierczak M, Zhang X, Chen B, Mulkey DK, Shi Y, Wagner PG, Pivaroff-Ward K, Sassic JK, Bayliss DA, Jegla T. (2013) External pH modulates EAG superfamily K+ channels through EAG-specific acidic residues in the voltage sensor. J Gen Physiol, 141 (6): 721-35. [PMID:23712551]

3. Miyake A, Mochizuki S, Yokoi H, Kohda M, Furuichi K. (1999) New ether-à-go-go K(+) channel family members localized in human telencephalon. J Biol Chem, 274 (35): 25018-25. [PMID:10455180]

4. Saganich MJ, Machado E, Rudy B. (2001) Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain. J Neurosci, 21 (13): 4609-24. [PMID:11425889]

5. Wulfsen I, Hauber HP, Schiemann D, Bauer CK, Schwarz JR. (2000) Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary. J Neuroendocrinol, 12 (3): 263-72. [PMID:10718922]

Contributors

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Citation information

K. George Chandy, Stephan Grissmer, George A. Gutman, Michel Lazdunski, David Mckinnon, Luis A. Pardo, Gail A. Robertson, Bernardo Rudy, Michael C. Sanguinetti, Walter Stühmer, Xiaoliang Wang.

Last modified on 07/10/2014.

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